no, just a reaction gif to what mistake he did
"You're trapped with a room filled with butthurt bronies."
"You're" means "You are"
In this case, he said "You are trapped with a room filled with butthurt bronies."
You may be mistaking "You're" with "Your", since "Your" means in posession of, such as "Your car." or "Your house is on fire."
An example of "You're" would be like "You're bad at this."